研究業績

1992 / 1993 / 1994 / 1995 / 1996 / 1997 / 1998 / 1999 / 2000 / 2001 / 2002 / 2003 / 2004 / 2005

2006 / 2007 / 2008 / 2009 / 2010 / 2011 / 2012 / 2013 / 2014 / 2015 / 2016 / 2017 / 2018 / 2019

2020 / 2021 / 2022 / 2023 / 2024

1999年

Certik, M., S. Shimizu. "Biosynthesis and regulation of microbial polyunsaturated fatty acid production." J. Biosci. Bioeng., 87(1), 1-14 (1999).

Certik, M., S. Shimizu. "Production and application of single cell oils." Agro-Food-Industry High-Tech, 10(3), 26-32 (1999).

Degen, O., M. Kobayashi, S. Shimizu, T. Eitinger. "Selective transport of divalent cations by transition metal permeases: The Alcaligenes eutrophus HoxN and the Rhodococcus rhodochrous NhlF." Arch. Microbiol., 171(3), 139-145 (1999).

藤川茂昭, 東山堅一, 清水昌. 油脂の発酵生産と生産菌の形態変化（目で見るバイオ）. バイオサイエンスとインダストリー, 57(12), 797-798 (1999).

藤川茂昭, 東山堅一, 清水昌. 糸状菌によるアラキドン酸含有油脂の発酵生産（解説）. バイオサイエンスとインダストリー, 57(12), 818-821 (1999).

Kamada, N., H. Kawashima, E. Sakuradani, K. Akimoto, J. Ogawa, S. Shimizu. "Production of 8,11-cis-eicosadienoic acid by a Δ5 and Δ12 desaturase-defective mutant derived from the arachidonic acid-producing fungus Mortierella alpina 1S-4." J. Am. Oil Chem. Soc., 76(11), 1269-1274 (1999).

Kataoka, M., K. Yamamoto, H. Kawabata, M. Wada, K. Kita, H. Yanase, S. Shimizu. "Stereoselective reduction of ethyl 4-chloro-3-oxobutanoate by Escherichia coli transformant cells co-expressing the aldehyde reductase and glucose dehydrogenase genes." Appl. Microbiol. Biotechnol., 51(4), 486-490 (1999).

Kita, K., T. Fukura, K. Nakase, K. Okamoto, H. Yanase, M. Kataoka. S. Shimizu. "Cloning, overexpression, and mutagenesis of the Sporobolomyces salmonicolor AKU4429 gene encoding a new aldehyde reductase, which catalyzes the stereoselective reduction of ethyl 4-chloro-3-oxobutanoate to ethyl (S)-4-chloro-3-hydroxybutanoate." Appl. Environ. Microbiol., 65(12), 5207-5211 (1999).

Kita, K., M. Kataoka, S. Shimizu. "Diversity of 4-chloroacetoacetate ethyl ester-reducing enzymes in yeasts and their application to chiral alcohol synthesis." J. Biosci. Bioeng., 88(6), 591-598 (1999).

Kita, K., K. Nakase, H. Yanase, M. Kataoka. S. Shimizu. "Purification and characterization of new aldehyde reductase from Sporobolomyces salmonicolor AKU4429." J. Mol. Catalysis B:Enzymatic, 6(3), 305-313 (1999).

Kobayashi, M., E. Sakuradani, S. Shimizu. "Genetic analysis of cytochrome b5 from arachidonic acid-producing fungus, Mortierella alpina 1S-4." J. Biochem., 125(6), 1094-1103 (1999).

Kobayashi, M., M. Goda, S. Shimizu. "Hydrazide synthesis: Novel substrate specificity of amidase." Biochem. Biophys. Res.Commun., 256(2), 415-418 (1999).

Kobayashi, M.,S. Shimizu. Cobalt proteins. Eur. J. Biochem., 261(1), 1-9 (1999).

小林達彦, 清水昌. スーパー生体触媒ニトリルヒドラターゼ: 重金属コバルトとのユニークな関係. 蛋白質核酸酵素, 44(1), 42-50 (1999).

小林達彦. スタンフォード・ソマビル研から. 生物工学誌, 77, 120 (1999).

小林達彦, 清水昌. ニコチン酸・ニコチンアミド. 日本臨牀, 57(10), 2211-2217 (1999).

Koga, S., J. Ogawa, Y.-M. Choi , S. Shimizu. "Coordinate induction of peroxidase and H₂O₂-forming nucleoside oxidase in Flavobacterium meningosepticum." FEMS Microbiol. Lett., 175(1), 113-117 (1999).

Koga, S., J. Ogawa, Y.-M. Choi, S. Shimizu. "Novel bacterial peroxidase without catalase activity from Flavobacterium meningosepticum: purification and characterization." Biochim. Biophys. Acta, 1435(2), 117-126 (1999).

Liu, J.-Q., M. Odani, T. Dairi, N. Itoh, S. Shimizu, H. Yamada. "A new route to l-threo-3-[4-(methylthio)phenylserine], a key intermediate for the synthesis of antibiotics: Recombinat low-specificity d-threonine aldolase-catalyzed stereospecific resolution." Appl. Microbiol. Biotechnol., 51(5), 586-591 (1999).

Nakano, N., N. Shirasaka, T. Murakami, S. Shimizu, H. Yoshizumi. "Formation of 8,11,14-octadecatrienoic acid (18:3 n-4) from naturally occurring unique fatty acid, 9,12-hexadecanoic acid (16:2 n-4), in animal cell cultures." J. Nutr. Sci. Vitaminol., 45, 203-212 (1999).

Ogawa, J., A. Ryono, S.-X. Xie, R.M. Vohra, R. Indrati, H. Miyakawa, T. Ueno, Y. Ikenaka, H. Nanba, S. Takahashi, S. Shimizu. "β-Carbon stereoselectivity of N-carbamoyl-d-α-amino acid amidohydrolase for α,β-diastereomeric amino acids." Appl. Microbiol. Biotechnol., 52(6), 797-801 (1999).

Ogawa, J., A. Ryono, S.-X. Xie, R.M. Vohra, R. Indrati, H. Miyakawa, T. Ueno, S. Shimizu. "Simultaneous recognition of two chiral centers in αβ-diastereomeric amino acids by N-carbamoyl-l-α-amino acid amidohydrolase from Alcaligenes xylosoxidans." Biotechnol Lett., 21(8), 711-713 (1999).

Ogawa, J., S. Shimizu. "Microbial enzymes: new industrial applications from traditional screening methods." Trends Biotechnol., 17 (1), 13-21 (1999).

Ogawa, J., S.-X. Xie, S. Shimizu. "Production of (R)-3-pentyn-2-ol through stereospecific hydrolysis of racemic 3-pentyn-2-ol esters with microbial enzymes." Appl. Microbiol. Biotechnol., 51(1), 53-57 (1999).

Ogawa, J., S.-X. Xie, S. Shimizu. "Stereoinversion of optically active 3-pentyn-2-ol by Nocardia species." Biotechnol. Lett., 21(4), 331-335 (1999).

Sakuradani, E., M. Kobayashi, S. Shimizu. "Δ9-Fatty acid desaturase from arachidonic acid-producing fungus: Unique gene sequence and its heterologous expression in a fungus, Aspergillus." Eur. J. Biochem., 260(1), 208-216 (1999).

Sakuradani, E., M. Kobayashi, T. Ashikari, S. Shimizu. "Identification of Δ12-fatty acid desaturase from arachidonic acid-producing Mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae." Eur. J. Biochem., 261(3), 812-820 (1999).

Sakuradani, E., M. Kobayashi, S. Shimizu. "Δ6-Fatty acid desaturase from an arachidonic acid-producing Mortierella fungus: Gene cloning and its heterologous expression in a fungus, Aspergillus." Gene, 238(2), 445-453 (1999).

Sakuradani, E., M. Kobayashi, S. Shimizu. "Identification of an NADH-cytchrome b₅ reductase gene from an arachidonic acid-producing fungus, Mortierella alpina 1S-4, by sequencing of the encoding cDNA and heterologous expression in a fungus, Apergillus oryzae." Appl. Environ. Microbiol., 65(9), 3873-3879 (1999).

清水昌. パントテン酸. 日本臨牀, 57(10), 2218-2222 (1999).

清水昌. 微生物によるビタミン類の製造研究. ビタミン, 73(12), 713-720 (1999).

Shimizu, S., M. Kataoka. Production of chiral C3- and C4-units by microbial enzymes, "Advances in Biochemical Engineering/Biotechnology, vol. 63, Biotransformations", Scheper, T., ed., Springer-Verlag, Berlin, p.109-123 (1999).

Shimizu, S., M. Kataoka. Aldehyde reductase, "Encyclopedia of Biprocess Technology: Fermentation, Biocatalysis, and Bioseparation", Flickinger, M. C. and S. W. Drew, eds., John Wiley & Sons, New York, p.63-69 (1999).

Shimizu, S., M. Kataoka. Lactonohydrolase, "Encyclopedia of Biprocess Technology: Fermentation, Biocatalysis, and Bioseparation", Flickinger, M. C. and S. W. Drew, eds., John Wiley & Sons, New York, p.1571-1577 (1999).

Shimizu, S., M. Kataoka. Pantothenic acid and related compounds, "Encyclopedia of Biprocess Technology: Fermentation, Biocatalysis, and Bioseparation", Flickinger, M. C. and S. W. Drew, eds., John Wiley & Sons, New York, p.1923-1934 (1999).

Shimizu, S., J. Ogawa. Oils, microbial production, "Encyclopedia of Biprocess Technology: Fermentation, Biocatalysis, and Bioseparation", Flickinger, M. C. and S. W. Drew, eds., John Wiley & Sons, New York, p.1839-1851 (1999).

Soong, C.-L., J. Ogawa, M. Honda, S. Shimizu. "Cyclic-imide-hydrolyzing activity of d-hydantoinase from Blastobacter sp. strain A17p-4." Appl. Environ. Microbiol., 65(4), 1459-1462 (1999).

Wada, M., H. Kawabata, M. Kataoka, Y. Yasohara, N. Kizaki, J. Hasegawa, S. Shimizu. "Purification and characterization of an aldehyde reductase from Candida magnoliae." J. Mol. Catalysis B:Enzymatic, 6(3) , 333-339 (1999).

Wada, M., H. Kawabata, A. Yoshizumi, M. Kataoka, S. Nakamori, Y. Yasohara, N. Kizaki, J. Hasegawa, S. Shimizu. "Occurrence of multiple ethyl 4-chloro-3-oxobutanoate-reducing enzymes in Candida magnoliae." J. Biosci. Bioeng., 87(2), 144-148 (1999).

Wada M, T. Matsumoto, S. Nakamori, M. Sakamoto, M. Kataoka, J.-Q. Liu, N. Itoh, H. Yamada, S. Shimizu. "Purification and characterization of a novel enzyme, l-threo-3-hydroxyaspartate dehydratase, from Pseudomonas sp. T62." FEMS Microbiol. Lett., 179(1), 147-151 (1999).

Wada, M., A. Yoshizumi, S. Nakamori, S. Shimizu. "Purification and characterization of monovalent cation-activated Levodione reductase from Corynebacterium aquaticum M-13." Appl. Environ. Microbiol., 65(10), 4399-4403 (1999).

Xie, S.-X., J. Ogawa, S. Shimizu. "NAD⁺-dependent (S)-specific secondary alcohol dehydrogenase involved in stereoinversion of 3-pentyn-2-ol catalyzed by Nocardia fusca AKU 2123." Biosci. Biotechnol. Biochem., 63(10), 1721-1729 (1999).

Xie, S.-X., J. Ogawa, S. Shimizu. "Production of (R)-3-pentyn-2-ol through stereoinversion of racemic 3-pentyn-2-ol by Nocardia fusca AKU 2123." Appl. Microbiol. Biotechnol., 52(3), 327-331 (1999).

Yasohara, Y., N. Kizaki, J. Hasegawa, S. Takahashi, M. Wada, M. Kataoka, S. Shimizu. "Synthesis of optically active ethyl 4-chloro-3-hydroxybutanoate by microbial reduction." Appl. Microbiol. Biotechnol., 51(6), 847-851 (1999).

Zhu, C., T. Ohashi, T. Morimoto, A.N. Onyango, K. Takao, S. Shiizu, S. Nakajima, N. Baba. "Synthesis of phospholipids bearing a conjugated oxo-polyunsaturated fatty acid residue." J. Chem. Research (S), 500-501 (1999).

1992 / 1993 / 1994 / 1995 / 1996 / 1997 / 1998 / 1999 / 2000 / 2001 / 2002 / 2003 / 2004 / 2005

2006 / 2007 / 2008 / 2009 / 2010 / 2011 / 2012 / 2013 / 2014 / 2015 / 2016 / 2017 / 2018 / 2019

2020 / 2021 / 2022 / 2023 / 2024